🔬 Dithiothreitol (DTT) — Overview Chemical Identity Name : 1,4-Dithiothreitol (DTT) Synonyms : Cleland’s Reagent Molecular Formula : C₄H₁₀O₂S₂ CAS Number : 3483-12-3 Molecular Weight : 154.25 g/mol Appearance : White crystalline powder Purity : ≥ 99% UV Absorbance : < 0.1 at 280 nm for a 1% solution Storage : Store at –20 °C Solubility : Highly soluble in water, ethanol, chloroform, ether, and ethyl acetate 🧪 Applications in Biochemistry & Molecular Biology Dithiothreitol (DTT) is a potent reducing agent widely utilized in various biochemical and molecular biology applications Reduction of Disulfide Bonds : DTT effectively reduces disulfide bonds in proteins and peptides, maintaining them in their reduced state. This property is crucial for protein denaturation and analysis. Protein Stabilization : By preserving thiol groups in their reduced form, DTT stabilizes enzymes and other proteins, preventing unwanted oxidati...